Product Name : Rutin 

Synonyms: glucopyranosyl]oxy]-2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4H-1-benzopyran-4-one; C.I. 75730

CAS No. 153-18-4

EINECS:205-814-1

Appearance:  yellow to greenish yellow crystalline powder

CAS No. 153-18-4

Molecular Formula: C₂₇H₃₀O₁₆^.3(H₂O)

Molecular Weight: 664.57

1. NF11

Identification: (A), (B), (C), (D), ( E ) positive reactions

Assay:  95.0%min

Loss on drying: 5.5-9.0%

Residue on ignition:  0.5%max

Chlorophyll:  0.004%max

Red pigment: 0.004%max

Quercetin: 0.5%max

Microbiological tests:

Total plate count/1g: 1000max

Yeast and mould/1g: 100max

E. Coil/1g: Absence

Package:  25kg/ fibre drum

1. EP9

First identification: B 

Second identification: A. C. D

-A (UV/ Vis absorption of methanol solution between 210nm and 450nm ):2maxima at 257nm and 358nm ,specific absorbance at 358nm is 305-330

-B (IR):Comparison : rutoside trihydrate CRS

-C (TLC): Principle spot is similar to that of reference solution

-D: Dissolve 10mg in 5ml of alcohol R, add 1g of Zn r and HCL R1,a red color develops .

Appearance: Yellow or greenish-yellow powder

Assay: 95.0%-101.0% (anhydrous substance)

Titration with 0.1M N(C4H9)4OH

Test:

Light-absorbing-impurities, at-Wave-length between 450nmand 800nm :0.10max

Substances insoluble in methanol: 3.0%max

Related substances:

Impurity A: Isoquercitroside :2.0%max

Impurity B: Kaempferol-3-rutinoside: 2.0%max

Impurity C:Quercetin :2.0%max

Any other impurity:0.1%max

Total:4.0%max

Water:7.5-9.5%

Sulphated ash: 0.1%max

Residual solvents:

   Methanol:3000ppm

   Acetone: Absence

   Propyl alcohol: Absence

Package: 25kg/ fibre drum